FIG. 2.

Immunoblot analysis of secreted and cell-associated AmyQ in cultures of the ecsA26 mutant, sup-15 suppressor mutant, and their wild-type parent. The upper panel shows secreted AmyQ in the medium of the cultures in Fig. 1 (the middle time point). The analyzed samples were in 3 μl of culture medium. The lower panel shows cell-associated amylase from cells in which P_xyn-amyQ was induced with 0.02% xylose at a cell density of 100 Klett units for 1 h. The samples are derived from 7.5 μl of the cultures. wt, wild type.