TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristics | Construction, source, or reference |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| SG38 | trpC2 amyE | 11 |
| JH642 | trpc2 phe-1 | Laboratory stock |
| SG38::pPS1326 | trpC2 amyE Ω(amyE::gpr"-"lacZ cat) | 56 |
| 2008 | trpC2 amyE Ω(amyE::gpr"-"lacZ cat) pRM113 | SG38::pPS1326 transformed to erythromycin resistance with pRM113 |
| 2009 | trpC2 amyE Ω(amyE::gpr"-"lacZ cat) pHT315 | SG38::pPS1326 transformed to erythromycin resistance with pHT315 |
| 2023 | trpC2 amyE yyaA::ermC | SG38 transformed to erythromycin resistance with pSG1443 |
| 2048 | trpC2 amyE pRM113 (multicopy plasmid) | SG38 transformed to erythromycin resistance with pRM113 |
| 2049 | trpC2 amyE pHT315 (multicopy plasmid) | SG38 transformed to erythromycin resistance with pHT315 |
| ts-134 | trpC2 thyA thyB dnaB-134(Ts) | 33 |
| JH21083 | trpC2 phe-1 amyE Ω(amyE::yyaA"-lacZ cat) | JH642 transformed to chloramphenicol resistance with pMB115 |
| JH21084 | trpC2 phe-1 spo0A12 amyE Ω(amyE::yyaA"-lacZ cat) | JH642 spo0A12 transformed to chloramphenicol resistance with pMB115 |
| JH21085 | trpC2 phe-1 spo0H81 amyE Ω(amyE::yyaA"-lacZ cat) | JH642 spo0H81 transformed to chloramphenicol resistance with pMB115 |
| JH21093 | trpC2 phe-1 abrB::Tn917erm amyE Ω(amyE::yyaA"-lacZ cat) | JH642 abrB::Tn 917 transformed to chloramphenicol resistance with pMB115 |
| JH21094 | trpC2 phe-1 amyE Ω(amyE::spoIIA"-lacZ cat) pHT315 | JH642 amyE::spoIIA"-lacZ transformed to erythromycin resistance with pHT315 |
| JH21095 | trpC2 phe-1 amyE Ω(amyE::spoIIA"-lacZ cat) pRM113 | JH642 amyE::spoIIA"-lacZ transformed to erythromycin resistance with pRM113 |
| JH21096 | trpC2 phe-1 amyE Ω(amyE::spoIIA"-lacZ cat) pHT315 | JH642 amyE::spoIIG"-lacZ transformed to erythromycin resistance with pHT315 |
| JH21097 | trpC2 phe-1 amyE Ω(amyE::spoIIG"-lacZ cat) pRM113 | JH642 amyE::spoIIG"-lacZ transformed to erythromycin resistance with pRM113 |
| E. coli | ||
| DH5α | F−endA1 hsdR17 supE44 thi-1 λ−recA1 gyrA96 relA1 Δ(lacZYA-argF)U169 φ80d lacZΔM15 | Gibco BRL |
| BL21(DE3)/pLysS | B F−dcm ompT hsdS(rB− mB−) gal λ(DE3) [pLysS] Camr | Promega |
| Plasmids | ||
| pET-3a | T7 expression vector | 51 |
| pET16b | T7 expression vector | Novagen |
| pJM116 | lacZ transcriptional fusion | 38 |
| pHT315 | Replicative vector (Ermr) | 3 |
| pBS19 | Replicative vector (Cmr) | 39 |
| pRM113 | pHT315 containing yyaA | This study |
| pSG250 | bla ermC | 9 |
| pSG1442 | pET-3a containing yyaA | This study |
| pSG1443 | ermC cassette from pSG250 inserted into SstI site of pSG1442 | This study |
| pMB2 | pET16b containing yyaA | This study |
| pMB115 | pJM116 containing yyaA promoter fused to lacZ | This study |