TABLE 2.
Sporulation efficiency of strains carrying multicopy plasmidsa
| Strain | No. of spores (cells counted) | % of spores relative to the wild type |
|---|---|---|
| SG38 (wild type) | 235 (300) | 100 |
| 2048 (YyaA overproduced) | 28 (314) | 11 |
| 2049 (control) | 128 (329) | 50 |
a
Samples of sporulating cells of strains SG38, 2048, and 2049 were taken 7 h after resuspension and fixed with ethanol as described in Materials and Methods. The numbers of phase-bright spores in fields of cells viewed by phase-contrast microscopy were scored. There was no lysis of 2048 and 2049 cells detected at this stage.