TABLE 1.
Bacterial strains and agr detection by PCR
| Strain | PCR agra | Nucleotide sequence denomination | GenBank accession no. | Strain | PCR agra | Nucleotide sequence denomination | GenBank accession no. | |
|---|---|---|---|---|---|---|---|---|
| S. aureus | ||||||||
| RN6911 | − | agr null | ||||||
| RN6390B | + | agr-1Sa | X52543 | |||||
| RN7690 | + | agr-1Sa | ||||||
| RN6923 | + | agr-2Sa | AF001782 | |||||
| RN9625 | + | agr-2Sa | ||||||
| RN8462 | + | agr-3Sa | AF001783 | |||||
| RN8463 | + | agr-3Sa | ||||||
| A880740 | + | agr-4Sa | AF288215 | |||||
| A890382 | + | agr-4Sa | ||||||
| S. arlettae DSM 20672 | + | agr-1ar | AF346712 | |||||
| S. auricularis | ||||||||
| ATCC 33753 | + | agr-1auri | AF346713 | |||||
| N910219 | + | agr-1auri | ||||||
| N910221 | + | agr-1auri | ||||||
| N910222 | + | agr-1auri | ||||||
| N910333 | + | agr-1auri | ||||||
| N910343 | + | agr-1auri | ||||||
| N940180 | + | agr-2auri | AF346714 | |||||
| N940327 | − | |||||||
| S. capitis subsp. capitis | ||||||||
| CCM 2734 | + | agr-1capi | AF346715 | |||||
| N900125 | + | agr-1capi | ||||||
| N930202 | + | agr-1capi | ||||||
| N930315 | + | agr-1capi | ||||||
| N940269 | + | agr-1capi | ||||||
| N950055 | + | agr-1capi | ||||||
| N910309 | + | agr-2capi | AF346716 | |||||
| N920161 | − | |||||||
| S. capitis subsp. ureolyticus ATCC 49326 | − | |||||||
| S. caprae | ||||||||
| N900362 | + | agr-1capra | AF346717 | |||||
| N9300039 | + | agr-1capra | ||||||
| N920271 | + | agr-2capra | AF346718 | |||||
| N930298 | + | agr-2capra | ||||||
| CCM3573 | − | |||||||
| N930422 | − | |||||||
| N920072 | − | |||||||
| N920021 | − | |||||||
| S. carnosus DSM 20501 | + | agr-1car | AF346719 | |||||
| S. chromogenes CCM 3387 | − | |||||||
| S. cohnii subsp. cohnii | ||||||||
| CCM 2736 | + | agr-1coco | AF346720 | |||||
| N900274 | + | agr-1coco | ||||||
| N920270 | + | agr-1coco | ||||||
| N930144 | + | agr-1coco | ||||||
| N940210 | + | agr-1coco | ||||||
| N940379 | + | agr-1coco | ||||||
| N940469 | + | agr-1coco | ||||||
| N920077 | − | |||||||
| N930306 | − | |||||||
| S. cohnii subsp. urealyticum ATCC 49330 | + | agr-1cour | AF346721 | |||||
| S. delphini DSM 20771 | − | |||||||
| S. epidermidis | ||||||||
| CCM 2124 | + | agr-1Se | Z49220 | |||||
| N910102 | + | agr-1Se | ||||||
| N910280 | + | agr-1Se | ||||||
| N910288 | + | agr-1Se | ||||||
| N910328 | + | agr-1Se | ||||||
| N910160 | + | agr-2Se | AF346724 | |||||
| N910173 | + | agr-2Se | ||||||
| N910191 | + | agr-3Se | AF346725 | |||||
| S. equorum DSM 20674 | − | |||||||
| S. felis ATCC 49168 | − | |||||||
| S. gallinarum CCM 3572 | + | agr-1gal | AF346722 | |||||
| S. haemolyticus CCM 2737 | − | |||||||
| S. hominis DSM 20328 | − | |||||||
| S. hyicus CCM 2368 | − | |||||||
| S. intermedius | ||||||||
| CCM 5739 | + | agr-1int | AF346723 | |||||
| HT20010088 | + | agr-1int | ||||||
| HT20010089 | + | agr-1int | ||||||
| HT20010090 | + | agr-1int | ||||||
| N900260 | − | |||||||
| N900269 | − | |||||||
| N900402 | − | |||||||
| N910201 | − | |||||||
| N930300 | − | |||||||
| N940276 | − | |||||||
| N940453 | − | |||||||
| N950082 | − | |||||||
| N950346 | − | |||||||
| S. kloosii DSM 20676 | − | |||||||
| S. lentus ATCC 29070 | − | |||||||
| S. lugdunensis | ||||||||
| RN8152 | + | agr-1Sl | AF173933 | |||||
| RN8153 | + | agr-1Sl | ||||||
| N940084 | + | agr-1Sl | ||||||
| N950245 | + | agr-1Sl | ||||||
| N960021 | + | agr-1Sl | ||||||
| N920217 | + | agr-2Sl | AF346728 | |||||
| N940118 | + | agr-2Sl | ||||||
| N940256 | + | agr-2Sl | ||||||
| N950199 | + | agr-2Sl | ||||||
| N960242 | + | agr-2Sl | ||||||
| S. muscae CCM 4175 | − | |||||||
| S. pasteuri ATCC 51129 | − | |||||||
| S. piscifermentans JCM 6057 | − | |||||||
| S. saprophyticus subsp. bovis CCM 4410 | − | |||||||
| S. saprophyticus subsp. saprophyticus CCM 883 | − | |||||||
| S. schleiferi subsp. coagulans JCM 7470 | − | |||||||
| S. schleiferi subsp. schleiferi ATCC 43808 | − | |||||||
| S. sciuri subsp. sciuri ATCC 29062 | − | |||||||
| S. simulans | ||||||||
| ATCC 27848 | + | agr-1sim | AF346726 | |||||
| N930284 | + | agr-1sim | ||||||
| N930309 | + | agr-1sim | ||||||
| N940200 | + | agr-1sim | ||||||
| N940232 | + | agr-1sim | ||||||
| N940279 | + | agr-1sim | ||||||
| N940377 | + | agr-1sim | ||||||
| N930143 | + | agr-2sim | AF346727 | |||||
| S. vitulinus ATCC 5145 | − | |||||||
| S. warneri CCM2 730 | − | |||||||
| S. xylosus | ||||||||
| ATCC 29971 | + | agr-1xyl | AF346729 | |||||
| N930189 | + | agr-1xyl | ||||||
| N930239 | + | agr-1xyl | ||||||
| N930408 | + | agr-1xyl | ||||||
| N930173 | − | |||||||
| N930249 | − | |||||||
| N930409 | − | |||||||
| N930409 | − |
a
Genomic DNA extracts were used as templates for PCR amplification with primers chosen to encompass a fragment of 1.2 kb of agr common to agr-1Sa to agr-3Sa, agr-1Se, and agr-1Sl as described in Materials and Methods. All strains tested were positive for PCR amplifying either gyrA or 16S-23S intergenic region.