FIG. 1.

(A) General structure of the lacZ reporter fusions constructed with Tn5lac. The horizontal line represents a segment of the M. xanthus chromosome. The box represents a transcription unit into which Tn5lac (wedge) has inserted. (B) Reporters (Ω numbers) are shown above arrows that indicate the time of gene expression during starvation-induced development (16). The dependence of each reporter on the A- and C-signals is shown below the time line by gray bars to indicate genes that are expressed normally in an asg (A-signal defective) or a csg (C-signal defective) mutant. Some of the insertions inactivate a known developmental function: Ω4408, sdeK; Ω4521, spi; Ω7540, fruA; Ω4414, dev; Ω7536, a spore shape function.