TABLE 2.
mRNA levelsa
| Time (h) | % Hybridization
|
||||||
|---|---|---|---|---|---|---|---|
|
fruA probe
|
devTRS probe
|
csgA probe
|
|||||
| act+ | ΔactB | act+ | ΔactB | act+ | ΔactB | ||
| 0 | 6 | 5 | 20 | 6 | 28 | 23 | |
| 8 | 100 | 87 | 100 | 39 | 100 | 41 | |
| 24 | 60 | 65 | 3 | 2 | 47 | 35 | |
a
mRNA was measured relative to the level of act+ (wild type) in Western slot blots. fruA mRNA was probed with a 123-bp fragment, AatII-KpnI, of the fruA gene (6). devTRS mRNA was probed with a 2.8-kbp HindIII-SalI fragment including most of devT and all of devR and devS (9). csgA mRNA was probed as described previously (9).