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. 2006 Jan 17;103(4):885–890. doi: 10.1073/pnas.0502470102

Fig. 4.

Fig. 4.

Effect of ghrelin on insulin content in MIN6 cells. (A) Cellular insulin concentration after 60-min incubation of MIN6 in the presence of 3.3 (□) or 22.2 (▪) mmol/liter glucose with 0.1, 1, 10 nmol/liter or without ghrelin. Cells were lysed with lysis buffer, and cellular insulin contents were assayed by ELISA and normalized by protein content of the lysate. (B) Insulin mRNA expression levels of MIN6 incubated with 0.01, 0.1, 1, or 10 nmol/liter or without ghrelin for 30 min. Data are expressed as fold increase relative to the values observed with nonstimulation. All values are expressed as the means ± SDs of three independent experiments (n = 6–12; *, P < 0.05 vs. nonstimulation with ghrelin).