Fig. 6.

Effects of Tof1p and Rrm3p on forks stalled by encounter between a RNA polymerase III transcription complex and a fork coming from the opposite direction. (Top) Diagram showing the structure of the plasmid pAT15B. (Middle and Bottom) Autoradiograms of 2D gels showing that encounter between transcription emanating from a tRNA promoter (by RNA pol III) and the fork moving counterclockwise from the ARS generate two elongated spots in WT cells (see parentheses and arrow); the spots are either missing or greatly attenuated in the Δtof1 and significantly enhanced in the Δrrm3 cells. Double deletion of the two genes (see Δrrm3Δtof1) restored the forks to a level significantly higher than that of the WT cells.