TABLE 6.
Opine induction of conjugal transfer is mediate only through the cognate traR gene of pAtK84b
Plasmid | Mutant traR | Frequency of transfer following growth witha:
|
|||
---|---|---|---|---|---|
Glucose | Nopaline | Agrocinopines A and B | Mannopine | ||
pAtK84b | None | <10−8 | 2 × 10−3 | 3 × 10−3 | <10−8 |
pPONocd | Noneb | <10−8 | <10−8 | 2 × 10−3 | <10−8 |
pPONtraRnoc | traRnoc | <10−8 | <10−8 | 3 × 10−3 | <10−8 |
pPONtraRacc | traRacc | <10−8 | 3 × 10−3 | <10−8 | <10−8 |
pPONtraRnoctraRacc | traRnoc, traRacc | <10−8 | <10−8 | <10−8 | <10−8 |
Donors were grown in ATN with glucose or glucose and the indicated opine. Conjugation frequencies are expressed as transconjugants recovered per input donor.
The mutation in ocd is polar on traRnoc; see text for details.