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Mutants of pAtK84b constitutive for nopaline catabolism are constitutive for conjugal transfer
| Plasmid | Transfer frequency following growth witha:
|
||
|---|---|---|---|
| Glucose | Nopaline | Agrocinopines A and B | |
| pAtK84b | <10−8 | 4 × 10−3 | 3 × 10−3 |
| pAtK84bnocc | 6 × 10−5 | 2 × 10−3 | 3 × 10−3 |
Donors were grown in ATN minimal medium containing glucose or glucose and the indicated opine. Conjugation frequencies are expressed as transconjugants recovered per input donor.
