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Effect of PAO on responses to hypoosmotic and hyperosmotic transfers. Loading and initial washout in the presence of 150 mM mannitol (M) before hypoosmotic shock (0 mM mannitol, C) is given at zero time on the graph. Control shock was given at 35 min of washout and PAO shock was given at 40 min of washout after the addition of 25 μM PAO at 20 min. Mannitol was readded after 45 min in hypoosmotic solution.
