Fig. 5.

Hypoosmotic efflux transient is independent of ABA. Shown are hypoosmotic transients in the presence and absence of ABA. The rate constant for efflux (h^–1) is plotted against time. Loading and initial washout in 0 mM mannitol was followed by transfer to 100 mM mannitol before or after an ABA-induced transient. Control, mannitol after 40 min and hypoosmotic transfer after 23 min in mannitol; ABA, ABA at 40 min, followed by transfer to mannitol at 97 min and hypoosmotic shock 22 min later; ABA*, ABA for the last 100 min of loading and throughout the washout, 40 min of washout in 0 mM mannitol, followed by transfer to mannitol for 23 min before hypoosmotic shock.