Fig. 1.

6q23-q24 DNA methylation profiling. (A) In silico digestion of the DNA sequence from 6q23-q24 identified five NotI and five AscI (N and A below the line) sites that migrate on an RLGS profile. Other NotI (n = 15) and AscI (n = 2) sites (N and A above the line) are too large or small for separation by RLGS. BACs containing the RLGS fragments are denoted with “RP11.” Known genes with corresponding RLGS fragments are indicated with arrows representing orientation. (B) RLGS quadrant depicting unmethylated AscI fragment 6E12 (arrow) in NSCLC patient 5 normal that is methylated in the paired tumor and A549 cell line. (C) Methylation percentages for each clone are denoted for NSCLC (black bars) and HNSCC (white bars).