Fig. 2.

COBRA and expression. (A) DNA methylation in paired normal and tumor samples by COBRA digestion by using BstUI in the bis1 PCR product. (B) Semiquantitative RT-PCR analysis of TCF21 on the samples assessed for DNA methylation by COBRA. Expression patterns were divided into categories of tumor equal to normal (T=N), less expression in tumor compared with normal (T<N) at either <5-fold or >5-fold, and tumor expression greater than normal (T>N). (C and D) Immunohistochemical staining by using an antibody against TCF21 on normal alveolar epithelium (C) demonstrating nuclear expression (arrows) and a non-small-cell carcinoma section (D) under ×40 magnification.