FIG. 1.

Schematic diagram of the junction between the left end of Tn4001T (thick line) and M. pulmonis genomic DNA (thin line) showing the location of the o.5, o.6, and o.8 primer binding sites (the arrows indicate directionality) used to determine the nucleotide position of the transposon in the genome by inverse PCR. The numbers refer to nucleotide positions, with position 1 being the first nucleotide of Tn4001T. The NlaIII site shown in M. pulmonis genomic sequences represents the NlaIII site that is closest to the left end of Tn4001T for the particular transformant being analyzed.