TABLE 3.

Summary of gene transfer experiments

Expt	Protocola	Parent		Parent 1 (CFU/ml)	Parent 2 (CFU/ml)	No. of progeny colonies	Presence or absenceb of:
		1	2				Mg2+	DNase I
1	Nat.	IVC-3 Tn4001C	KD-T2 Tn916	2.2 × 108	1.1 × 108	4	−	−
2	Nat.	IVC-3 Tn4001C	KD-T2 Tn916	2.2 × 108	6 × 109	2	−	−
3	Nat.	IVC-3 Tn4001C	KD-T2 Tn916	4 × 108	8 × 108	9	−	−
3	Nat.	IVC-3 Tn4001C	KD-T2 Tn916	4 × 108	8 × 108	8	−	+
4	Nat.	IVC-3 Tn4001C	KD305 Tn4001T	6.0 × 108	1.3 × 109	10	−	−
5	Nat.	IVC-3 Tn4001C	KD305 Tn4001T	NDc	ND	2	−	−
6	Nat.	CT186 Tn4001T	IVC-3 Tn4001C	ND	ND	8	−	−
6	Nat.	CT186 Tn4001T	IVC-3 Tn4001C	ND	ND	9	−	+
7	Nat.	CT182 Tn4001T	IVC-3 Tn4001C	7.0 × 106	1.8 × 109	7	−	−
8	Nat.	CT247 Tn4001T	IVC-3 Tn4001C	3.0 × 107	2.7 × 109	5	−	−
9	Nat.	CT G5 Tn4001C	KD-T2 Tn916	6.0 × 106	1.0 × 108	11	−	−
10	50% PEG 3350	IVC-3 Tn4001C	KD-T2 Tn916	ND	ND	1,100	−	−
10	50% PEG 8000	IVC-3 Tn4001C	KD-T2 Tn916	ND	ND	700	−	−
11	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	6 × 107	6 × 107	>3,000	−	−
11	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	6 × 107	6 × 107	>3,000	+	−
11	5% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	6 × 107	6 × 107	0	−	−
11	5% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	6 × 107	6 × 107	0	+	−
12	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	ND	ND	96	−	−
12	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	ND	ND	80	+	−
13	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	2.5 × 107	4.0 × 107	>3,000	−	−
13	50% PEG 3350	CT186 Tn4001T	IVC-3 Tn4001C	2.5 × 107	4.0 × 107	>3,000	−	+
14	50% PEG 3350	CT182 Tn4001T	IVC-3 Tn4001C	4.5 × 106	8.5 × 107	2	−	−
15	50% PEG 3350	CT247 Tn4001T	IVC-3 Tn4001C	1.6 × 105	1.2 × 108	16	−	−

^aMating was done by the natural broth method (Nat.) or was PEG mediated at the indicated percentage and type of PEG.

^bThe presence (+) or absence (−) of Mg²⁺ and DNase I is shown.

^cND, not determined.