TABLE 4.
PCR analysis of KD × CT matings and the direction of gene transfera
| Mating | Total no. of progeny analyzed | No.
|
|
|---|---|---|---|
| KD to CTb | CT to KDc | ||
| IVC-3 × CT182 (Nat.) | 6 | 0 | 6 |
| IVC-3 × CT182 (PEG) | 3 | 1 | 2 |
| IVC-3 × CT186 (Nat.) | 8 | 0 | 8 |
| IVC-3 × CT186 (PEG) | 3 | 0 | 3 |
| IVC-3 × CT247 (Nat.) | 5 | 0 | 5 |
| IVC-3 × CT247 (PEG) | 15 | 10 | 5 |
a
The primer pairs for these PCR experiments are presented in Table 2.
b
CT recipients were each PCR positive for cat (primer pair 4), tetM (pair 3), and lipB (pair 1) and retained the original Tn4001T-mycoplasmal genome junction of the CT parent (pair 5, 6, or 7, as appropriate). CT recipients were PCR negative for p93 (pair 2).
c
KD recipients were each PCR positive for cat, tetM, and p93 and lacked the Tn4001T-mycoplasmal genome junction found in the CT parent. KD recipients were PCR negative for lipB.