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Degradation of OHHL in conditioned medium. Synthetic OHHL (2 μM) and chloramphenicol (50 μg/ml) were added to jbC1 culture supernatants which had been harvested throughout the growth curve. The mixtures were incubated for 18 h at 30°C before measurement of the OHHL concentration (▴) and pH (▵) of the samples. Error bars represent the standard error of the mean from two independent cultures.
