Skip to main content
. Author manuscript; available in PMC: 2026 Aug 18.
Published in final edited form as: Differentiation. 2026 Jun 7;150:100973. doi: 10.1016/j.diff.2026.100973

Fig. 1. Cdx, Foxb, and Sox1/2/3 loci and sgRNA validation results.

Fig. 1.

Selected sgRNA target sites are indicated, along with Amplicon-EZ (Genewiz) indel plots and estimated mutagenesis rates. Red arrows indicate CRISPR-generated indel peaks. Grey asterisks indicate naturally-occurring indels. Mutagenesis rates are automatically calculated by the Amplicon-EZ service where only one major indel peak is present. Rates are approximated for plots in which naturally occurring indels confounded automatic rate estimation. In such cases, the mutagenesis rate is at least higher than the height of the CRISPR indel peak.