Skip to main content
. Author manuscript; available in PMC: 2026 Aug 18.
Published in final edited form as: Differentiation. 2026 Jun 7;150:100973. doi: 10.1016/j.diff.2026.100973

Fig. 2. Dmbx, Engrailed, and Mnx loci and sgRNA validation results.

Fig. 2.

Selected sgRNA target sites are indicated, along with Amplicon-EZ (Genewiz) indel plots and automated mutagenesis rate calculations. Red arrows indicate CRISPR-generated indel peaks. Grey asterisks indicate naturally-occurring indels. Mutagenesis rates are approximated for plots in which naturally occurring indels confounded automatic rate estimation as described before.