Fig. 5. Plotting predicted and measured mutagenesis efficacies.

Plots comparing predicted efficacy scores (Doench ’16, Doench Ruleset 3/RS3, or normalized/averaged Doench scores) to actual mutagenesis efficacies as measured/estimated by Amplicon-EZ sequencing. All data according to the table in Fig. 4. Pearson’s correlations calculated and shown for each set of predictions.