FIG. 3.

3O-C₁₂-HSL is a potent inducer of proinflammatory cytokines and chemokines in vivo. RNA was extracted from the skin of mice injected with PBS or 10 μM 3O-C₁₂-HSL and was used in RNase protection assays to identify induction of inflammatory chemokines (A) or cytokines (B). Induction was quantified by densitometry and standardized to internal GAPDH controls. Graphs show the mean fold induction compared to PBS controls. Each bar is the mean from samples from four separate mice. Error bars indicate standard deviations.