FIG. 5.

3O-C₁₂-HSL induces Cox-2 mRNA and protein in the skin of mice. RNA and protein were extracted from the skin of mice injected with PBS or 3O-C₁₂-HSL. (A) Agarose gel showing ethidium bromide-stained RT-PCR products specific for Cox-1 and Cox-2. Lanes 1 and 2, samples from two separate mice injected with PBS; lanes 3 and 4, samples from two mice injected with 10 μM 3O-C₁₂-HSL. (B) Proteins from the skin of mice injected with PBS or 3O-C₁₂-HSL were evaluated in a Western blot specific for Cox-2. This is a representative blot from four separate experiments.