FIG. 5.

Northern blot analysis of cpeBA, cpeCDE, and cpcB2A2 expression in WT, the FdBk mutant, and the FdTq1 mutant. (A) Representative autoradiograms of the data presented in panels B to D showing hybridization to the probes listed on the left. RNA was isolated from the WT and the two mutant lines after growth in 15 μmol of RL and GL m⁻² s⁻¹. (B) Mean values from four independent experiments of cpeBA RNA, expressed as a percentage of the WT GL value (set to 100%). (C) Mean values from three independent experiments of cpeCDE RNA, expressed as a percentage of the WT GL value (set to 100%). (D) Mean values from four independent experiments of cpcB2A2 RNA, expressed as a percentage of the WT RL value (set to 100%). The numbers in parentheses are the fold induction between RL and GL for each operon in each cell line. Standard errors are shown; P values are provided in the text. Measurements were normalized by using ribosomal values prior to calculation of means.