FIG. 5.

Identification of mycobacterial 16S rRNA gene sequences and their linkage with murA and tyrS genes. Mycobacterial DNA was digested with BamHI, and the fragments were separated by electrophoresis through 0.75% (wt/vol) agarose and transferred to a nylon membrane. ³²P-labeled probes were used to identify murA (A), 16S rRNA gene (B), and tyrS (C) sequences. Fragment sizes are shown on the left. The mycobacterial DNA samples were isolated from M. agri (lane 1), M. alvei (lane 2), M. diernhoferi (lane 3), M. fortuitum (lane 4), M. mucogenicum (lane 5), M. peregrinum (lane 6), M. porcinum (lane 7), M. senegalense (lane 8), and M. wolinskyi (lane 9). The dotted lines link radioactive bands of identical sizes.