FIG. 1.

Autoradiographs of Northern blots probed with O₂-induced RAPs. Mid-log-phase cultures of wild-type strain 638R (W⁺) and strain IB263 [oxyR(Con)] were grown anaerobically (−) or stressed by aerobic incubation for 1 h (+). (A to E) Probing with the RAP products; (F) probing with an internal katB fragment; (G) probing with an internal sod fragment. All lanes contain 50 μg of total RNA except for those in panel A, which contain 80 μg/lane. Approximate sizes of the hybridizing transcripts in kilobases are shown and were extrapolated from a molecular weight ladder in an adjacent lane.