TABLE 3.
Verification of differentially expressed genes in the transcriptome by QRT-PCR.
| KEGG pathway | Gene ID | Gene | Symbol | KO | log2 (FC)value * | P-adj | 2–ΔΔCT ± SD by HIF treated samples | 2–ΔΔCT ± SD of control samples |
|---|---|---|---|---|---|---|---|---|
| Plant-pathogen interaction | LOC102606712 | cngic1 | CNGC (cyclic nucleotide channel, plant) | K05391 | 7.26 up | 6.15e-08 | 4.52 ± 0.52 up | 1.19 ± 0.50 |
| Plant-pathogen interaction | LOC102609173 | mapkkk1 | MEKK1 (mitogen-actived protein kinase kinase kinase 1) | K13414 | 3.03 up | 3.09e-18 | 3.81 ± 0.96 up | 1.82 ± 0.68 |
| Phenylpropanoid metabolism | LOC112495556 | paA4g1 | E3.5.1.4, amiE | K01426 | 3.94 up | 1.59e-07 | 6.23 ± 0.34 up | 1.66 ± 0.32 |
| Phenylpropanoid metabolism | LOC102625005 | paA4g2 | E3.5.1.4, amiE | K01426 | 1.46 up | 0.04 | 5.81 ± 0.96 up | 1.80 ± 0.60 |
*P < 0.05 and |log2(foldchange)| > 1 were used as the criteria for screening DEG. E3.5.1.4, paA4g1 was amiE (Acetamide hydrolase), it is involved in the carbon and nitrogen metabolism balance and affects the formation of biological membranes.