TABLE 1.

Quantitation of Northern blot analyses of mini-tet(L) mRNA decay^a

Plasmid	Description of leader region	Half-life (min)		Fold increase in half-life
		Without Tc	With Tc
pYW101	Wild type	17.2 ± 2.3	41.8 ± 2.9	2.5
pYW107	RBS2 mutation	18.0 ± 7.8	34.0 ± 5.7	1.9b
pYW106	Deletion of codons 13-15	15.5 ± 0.7	35.0 ± 7.1	2.3
pYW104	Stop at codon 2	20.5 ± 2.1	62.7 ± 6.3	3.0
pYW102	Stop at codon 5	27.3 ± 3.9	51.0 ± 12.7	1.9b
pYW105	Stop at codon 12	22.5 ± 0.5	50.5 ± 9.1	2.2
pYW103	Mutated initiation codon	7.4 ± 0.4	26.8 ± 9.0	3.6
pYW109	RBS1 mutation	3.2 ± 0.2	3.6 ± 0.1	1.2b

^aFor all Northern blot analyses, data are from experiments in which the linear regression gave an R² value of >0.90. Data are the averages of three experiments, except where indicated.

^bAverage of two experiments.