TABLE 1.
Ca2+-DPA- and nutrient-induced germination of spores of various strainsa
| Inducer | Strain (genotype) | Amt of spores at an OD600 of 1 (CFU/ml) |
|---|---|---|
| Ca2+-DPA | FB104 (ger3) | 1.9 × 108 |
| IB652 (ger3 cwlJ-His-tag) | 2.0 × 108 | |
| FB115 (ger3 cwlJ) | 7.0 × 106 | |
| Nutrients | FB110 (sleB) | 1.2 × 108 |
| IB664 (sleB cwlJ-His-tag) | 1.0 × 108 | |
| FB113 (sleB cwlJ) | <105 |
a
Spore germination with Ca2+-DPA or nutrients was assessed as described in the text. Note that wild-type (strain PS832) spores routinely give 1×108 to 2×108 CFU in these assays (14, 16).