ISHp608 insertions in F factor in E. coli. Termini and sites of ISHp608cam insertions in F factor DNA (GenBank accession no. NC002483). Left and right termini of ISHp608s are shown in uppercase and flanking DNAs are shown in lowercase letters. The products of transposition from donor plasmid 3 (A+B−) (eight sites of insertion) were as follows: 2-1, position 60704/5; 5-2, position 62725/6; 6-1, position 64675/6; 2-2, position 65422/3; 1-1, position 80949/50; 5-1, position 85934/5; 6-2, position 86796/7; and 1-2, position 99/100. The products of transposition from donor plasmid 4 (A+B+) (five sites of insertion) were: 18-1, position 65422/3; 18-2 and 23-1 each at (although independent), position 95172/3; 23-2, position 59288/9; and 24-1, position 70860/1.