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Hoechst 33342 transport in everted membrane vesicles energized by ATP (A) or lactate (B). Sequential additions of substances are indicated by arrows: a, 0.2 μM Hoechst 33342; b, 0.1 mM ATP (panel A) or 0.5 mM lactate (panel B); c, 1.8 μM valinomycin; and d, 2.7 μM nigericin. Dotted lines indicate control vesicles. Solid lines indicate NorA-His-containing vesicles. A value of 100% was defined as maximal Hoechst 33342 binding before ATP or lactate was added.
