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. 2002 Mar;184(5):1359–1369. doi: 10.1128/JB.184.5.1359-1369.2002

FIG. 7.

FIG. 7.

Effect of a fis mutation on the growth rate regulation of the amount of the pdxA-ksgA cotranscript, the amount of the ksgA- and pdxB-specific transcripts, and the total amount of the ksgA transcript. Total RNA isolated from NU426 (open symbols) or TX3333 (NU426 fis) (closed symbols) growing exponentially in minimal salts-acetate medium, MMGly, or EMMG was probed as described in Materials and Methods. Counts per minute corresponding to individual bands were expressed as the amount of mRNA per gene (see Materials and Methods), and the average amount of mRNA per gene for each transcript was calculated from three and four independent experiments for NU426 and TX3333, respectively, and plotted against the growth rate. Standard deviations of the mean are shown. Panels: A, pdxA-ksgA cotranscript; B, ksgA-specific transcript; C, total ksgA transcript (sum of panels A and B); D, pdxB-specific transcript.