TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or reference |
|---|---|---|
| Strains | ||
| AL0875 | thi-1 leu6 lacY1 lacIZ Δ(mlu) supE44 tonA21 rpsL rfbD1 lipB::mini-Tn10 | 54 |
| CAG12093 | MG1655 car-96::Tn10 | 62 |
| CF1693 | MG1655 ΔrelA251::kan ΔspoT207::cat; Cmr Kmr | M. Cashel collection |
| JC7623 | arg ara his leu pro recB21 recC22 sbcB15 thr | 55 |
| RJ1529 | MG1655 fis::767 Δ(lac-pro) ara str | 5 |
| NK7049 | ΔlacX74 galOP308 rpsL Smr | 61 |
| NU426 | W3110 prototroph | C. Yanofsky collection |
| NU816 | Single colony isolate of W3110 tnaA2 ΔlacU169 | C. Yanofsky collection |
| NU1187 | NU816 pdxA::mini-Mud-1-1 Kmr | 55 |
| NU1700 | JC7623 pdxB::mini-MudII Kmr | Transformation with linearized pNU250; this study |
| NU1702 | NU816 pdxB::mini-MudII Kmr | NU816 × P1 kc NU1700 |
| TX1892 | NU816 ΔrelA251::kan; Kmr | NU816 × P1 kc (CF1693) This study |
| TX2093 | NU1187(pNO2661, pNO2696) | This study |
| TX2094 | NU1187(pNO1301) | This study |
| TX2095 | NU1187(pNO1302) | This study |
| TX2096 | NU1187(pBR322) | This study |
| TX2169 | NU1187(pNU122) | This study |
| TX2171 | NU1187(pUC19) | This study |
| TX2243 | NU1187(pBR322, pACYC184) | This study |
| TX2254 | NU1187(pNU276) | This study |
| TX2269 | NU1187(pPR274) | This study |
| TX2283 | NU1702(pTX291) | This study |
| TX2357 | NU1187(pTX288) | This study |
| TX2737 | NU426(pALS13) | This study |
| TX2739 | NU426(pALS14) | This study |
| TX3333 | NU426 fis::767 | NU426 × P1 vir (RJ1529) |
| TX3338 | NU426 lipB::mini-Tn10; Tcr | NU426 × P1 vir (AL0875) |
| TX4080 | NK7049 with pdxJ-lacZ protein fusion λRS641; Smr | This study |
| TX4082 | NK7049 with pdxJ-lacZ protein fusion λRS643; Smr | This study |
| Plasmids | ||
| pACYC184 | Replicon P15A; Cmr Tcr | 46 |
| pALS13 | Ptac::relA" (445 N-terminal amino acids of relA) lacIq | 64 |
| pALS14 | Ptac::relA" (331 N-terminal amino acids of relA) lacIq, Apr | 64 |
| pBR322 | Replicon ColE1; Apr Tcr | 46 |
| pGEM3Z | pUC18 derivative with phage T7 and SP6 promoters | Promega |
| pNO1301 | pBR322 (rrnB+); Apr | 33 |
| pNO1302 | pNO1301 with 2.45-kb deletion in rrnB; Apr | 33 |
| pNO2661 | pACYC184 (RNA polymerase β and β") Cmr | 8 |
| pNO2696 | pBR322 (RNA polymerase α and σ70) Apr | 8 |
| pNU122 | pUC19 (pdxA+ksgA+apaG+apaH+); Apr | 55 |
| pNU180 | pGEM3Z (386-bp pdxA-ksgA region); Apr | 59 |
| pPR274 | mini-F" replicon; CmrtrpE+ | 47 |
| pNU276 | pBR322 (pdxA+ plus upstream region) 5.0-kb EcoRI fragment from Kohara phage clone 8D2; Apr | This study |
| pTX288 | pPR274 (pdxA+) same insert as plasmid pNU276; Cmr | This study |
| pTX291 | pPR274 (pdxB+) 4.4-kb insert containing entire pdxB operon; Cmr | This study |
| Phage λ-borne lacZ fusions | ||
| λRS641 | Δrnc-36 era+recO+pdxJ"::lacZ" | 45 |
| λRS643 | Δrnc-76 Δrnc-36 era+recO+pdxJ"::"lacZ | 45 |
a
Apr, Cmr, Kmr, and Tcr indicate resistance to ampicillin, chloramphenicol, kanamycin, and tetracycline, respectively.