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. 2002 Mar;184(5):1234–1243. doi: 10.1128/JB.184.5.1234-1243.2002

FIG. 1.

FIG. 1.

Purification and factor Xa cleavage of MBP-MrsD fusion protein. (A) Purification and factor Xa cleavage of MBP-MrsD was followed by SDS-PAGE. Lanes: M, molecular mass marker (sizes are in kilodaltons in the left margin); 1 and 2, whole-cell lysate of E. coli TB1(pIH902 mrsD) before and after induction with IPTG; 3, cleared cell lysate; 4, MBP-MrsD purified by amylose affinity chromatography; 5 to 8, cleavage of MBP-MrsD with factor Xa after 1, 2, 3, and 17.5 h of incubation at room temperature, respectively. (B) Absorption spectra of purified MBP-MrsD (black line) and purified MBP-EpiD (gray line).