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. 2002 Mar;184(5):1234–1243. doi: 10.1128/JB.184.5.1234-1243.2002

FIG. 2.

FIG. 2.

Purification of MrsD and MrsD H75N. Purification of MrsD (upper part of the figure) and MrsD H75N (lower part of the figure) was followed by SDS-PAGE (A) and immunoblotting (B). Lanes: L and H, low- and high-molecular-mass markers (sizes are in kilodaltons in the left and right margins); 1 and 2, whole-cell lysate of E. coli M15(pREP4)(pQE12 mrsD or pQE12 mrsD H75N) before and after induction with IPTG; 3, cleared cell lysate; 4, HiTrap Q-enriched proteins; 5, MrsD or MrsD H75N purified by gel filtration by using a Superdex 200 PC 3.2/30 column.