TABLE 2.
Plasmid complementation of M. acetivorans proABC mutationsa
| Mutation | Complementation with: | |||
|---|---|---|---|---|
| Vector only | proABC | proAB | proA | |
| pPB35 | pPB36 | None | pJK76 | |
| proA1::Tn5-407 | − | + | NDb | + |
| proA2::Tn5-407 | − | + | ND | + |
| proB1::Tn5-407 | − | + | ND | ND |
| proC2::pac-ori-aph | − | + | ND | ND |
| proC1::aph-ori-pac | − | + | ND | ND |
| pWM321 | pJK72 | pJK74 | pJK75 | |
| proA3::Tn5-ileS12 | − | + | + | + |
| proA4::Tn5-ileS12 | − | + | + | + |
| proB2::Tn5-ileS12 | − | + | + | ND |
| proB3::Tn5-ileS12 | − | + | + | ND |
| proC3::Tn5-ileS12 | − | + | ND | ND |
| proC4::Tn5-ileS12 | − | + | ND | ND |
a
Complementation was scored according to ability to grow on HA-MA medium with puromycin and pseudomonic acid but without proline. Plasmids used are described in the text and shown in Fig. 1.
b
ND, not done.