TABLE 2.
Germination survey for B. anthracis of major naturally utilized amino acids with inosine
| Germinanta,c | % Germination in 15 minb for:
|
|
|---|---|---|
| Parental strain | ΔgerS strain | |
| Inosine | 5.6 ± 1.3 | 4.5 ± 1.3 |
| Inosine/alanine | 73.1 ± 1.5 | 51.0 ± 6.7 |
| Inosine/cysteine | 46.3 ± 1.9 | 2.7 ± 1.8 |
| Inosine/histidine | 64.4 ± 4.1 | 1.9 ± 0.8 |
| Inosine/methionine | 77.0 ± 6.3 | 2.3 ± 1.0 |
| Inosine/phenylalanine | 74.4 ± 4.0 | 2.4 ± 0.6 |
| Inosine/proline | 72.6 ± 3.0 | 2.5 ± 1.2 |
| Inosine/serine | 84.5 ± 8.6 | 3.7 ± 0.3 |
| Inosine/tryptophan (1 mM) | 79.1 ± 2.9 | 2.8 ± 0.7 |
| Inosine/tyrosine (1 mM) | 74.1 ± 2.1 | 2.1 ± 0.5 |
| Inosine/valine | 62.4 ± 4.3 | 2.2 ± 0.8 |
a
All amino acids are l-isomers at 100 mM, unless otherwise noted. Inosine is at 1 mM.
b
Results are the average of triplicate experiments on two independent preparations with 1 standard deviation.
c
The following amino acids were tested (100 mM each) in conjunction with inosine (1 mM) but were found not to alter the percent germination significantly above the inosine (1 mM) baseline: arginine, asparagine, aspartate, glutamate, glutamine, glycine, isoleucine, leucine, lysine, and threonine.