TABLE 3.
Effect of gerS mutation on high-concentration solution kinetics in B. anthracis ΔSterne 34F2
| Germinantb | Maximum Gia (% germination/min)
|
Score for 16-h germinationc
|
||
|---|---|---|---|---|
| Parental | ΔgerS | Parental | ΔgerS | |
| Alanine | 6.1 ± 0.4 | 7.3 ± 0.9 | + | + |
| Alanine/histidine | 6.7 ± 1.8 | 6.2 ± 0.5 | + | + |
| Alanine/proline | 9.1 ± 2.1 | 7.2 ± 0.7 | + | + |
| Alanine/tryptophan (1 mM) | 11.0 ± 1.5 | 6.3 ± 0.5 | + | + |
| Alanine/tyrosine (1 mM) | 10.1 ± 1.1 | 7.4 ± 0.6 | + | + |
| Inosine | <0.1 | <0.1 | − | − |
| Inosine/alanine | 16.5 ± 1.9 | 7.2 ± 1.0 | + | + |
| Inosine/cysteine | 10.0 ± 0.3 | <0.1 | + | + |
| Inosine/histidine | 3.5 ± 1.1 | <0.1 | + | − |
| Inosine/methionine | 6.8 ± 0.5 | <0.1 | + | − |
| Inosine/phenylalanine | 7.5 ± 0.9 | <0.1 | + | − |
| Inosine/proline | 8.9 ± 2.2 | <0.1 | + | − |
| Inosine/serine | 10.5 ± 1.3 | <0.1 | + | + |
| Inosine/tryptophan (1 mM) | <0.1 | <0.1 | + | − |
| Inosine/tyrosine (1 mM) | 0.8 ± 0.1 | <0.1 | + | − |
| Inosine/valine | 5.8 ± 1.3 | <0.1 | + | − |
a
The average of triplicate experiments of two spore preparations is shown with 1 standard deviation.
b
All amino acids are at 100 mM, except tyrosine and tryptophan, which are at 1 mM. Inosine is at 50 mM.
c
Samples were done in triplicate and scored as germinating (+, >10% germination) or nongerminating (−, ≤10% germination) after 16 h.