TABLE 4.
Effect of gerS mutation on dilute solution kinetics in B. anthracis ΔSterne 34F2
| Germinantb | Maximum germination ratea (% germination/min)
|
Score for 16-h germination of mutantc
|
||
|---|---|---|---|---|
| Parental | ΔgerS | Parental | ΔgerS | |
| Alanine | <0.1 | <0.1 | + | + |
| Alanine/histidine | 1.8 ± 1.6 | <0.1 | + | + |
| Alanine/proline | 4.9 ± 1.9 | <0.1 | + | + |
| Alanine/tryptophan | 1.8 ± 0.1 | <0.1 | + | + |
| Alanine/tyrosine | 2.4 ± 0.7 | <0.1 | + | + |
| Inosine | <0.1 | <0.1 | − | − |
| Inosine/alanine | 12.9 ± 0.9 | <0.1 | + | + |
| Inosine/cysteine | <0.1 | <0.1 | + | − |
| Inosine/histidine | 6.0 ± 0.6 | <0.1 | + | − |
| Inosine/methionine | 7.4 ± 0.9 | <0.1 | + | − |
| Inosine/phenylalanine | 10.2 ± 0.4 | <0.1 | + | − |
| Inosine/proline | <0.1 | <0.1 | + | − |
| Inosine/serine | 7.9 ± 1.5 | <0.1 | + | + |
| Inosine/tryptophan | 4.6 ± 0.1 | <0.1 | + | − |
| Inosine/tyrosine | 7.0 ± 0.4 | <0.1 | + | − |
| Inosine/valine | 1.3 ± 0.7 | <0.1 | + | − |
a
The average of triplicate experiments of two spore preparations is shown with 1 standard deviation.
b
All amino acids and inosine are at 1 mM.
c
Samples were done in triplicate and scored as germinating (+, >10% germination) or nongerminating (−, ≤10% germination) after 16 h.