TABLE 5.
Effects of d-alanine on germination of B. anthracis ΔSterne 34F2
| Germinant (1 mM each) | Germination in 30 mina with:
|
||
|---|---|---|---|
| No d-alanine | 1 mM d-alanine | 10 mM d-alanine | |
| Alanine | + | − | − |
| Alanine/histidine | + | − | − |
| Alanine/proline | + | − | − |
| Alanine/tryptophan | + | − | − |
| Alanine/tyrosine | + | − | − |
| Inosine | − | − | − |
| Inosine/alanine | + | + | − |
| Inosine/cysteine | + | + | − |
| Inosine/histidine | + | + | + |
| Inosine/methionine | + | + | − |
| Inosine/phenylalanine | + | + | + |
| Inosine/proline | + | + | − |
| Inosine/serine | + | + | − |
| Inosine/tryptophan | + | + | + |
| Inosine/tyrosine | + | + | + |
| Inosine/valine | + | + | − |
a
Samples were done in triplicate and scored as germinating (+, >10% germination) or nongerminating (−, ≤10% germination) at 30 min. All amino acids are l-isomers unless stated. The buffer and reaction conditions are standard.