TABLE 6.

Effect of aromatic side chains on germination in B. anthracis

Germinanta	Maximum Gi (% germination/min)b		Extent of germination after 15 min (% germination)b
	Parental	ΔgerS	Parental	ΔgerS
10 mM alanine	1.4 ± 0.2	1.2 ± 0.4	6.5 ± 1.2	6.2 ± 1.0
10 mM alanine/1 mM tyrosine	6.9 ± 0.5*	1.6 ± 0.5	61.0 ± 5.6†	6.0 ± 0.8
10 mM alanine/1 mM phenol	2.9 ± 0.9*	1.7 ± 0.4	26.3 ± 3.6†	7.1 ± 0.9
10 mM alanine/1 mM tryptophan	4.7 ± 0.8*	1.9 ± 0.8	56.0 ± 5.4†	6.3 ± 2.0
10 mM alanine/1 mM indole	2.4 ± 0.3*	1.3 ± 1.0	33.2 ± 4.5†	7.5 ± 2.0
10 mM alanine/1 mM inosine	8.2 ± 0.9*	1.4 ± 0.6	73.0 ± 9.5†	10.5 ± 0.9
10 mM alanine/1 mM hypoxanthine	0.6 ± 0.2*	1.0 ± 0.2	6.0 ± 0.2	6.0 ± 0.5

^aAll amino acids are l-isomers; all solutions were done in the standard buffer.

^bResults are the average of triplicate experiments on two independent preparations with 1 standard deviation. *, result is statistically different from the alanine baseline, P < 0.01. ^†, result is statistically different from that for the ΔgerS mutant, P < 0.01.