FIG. 5.

Signal transduction from TorS of E. coli to TorR of S. oneidensis. (A) Effect of E. coli and S. oneidensis response regulator TorR on torE"-lacZ fusion expression in E. coli. Strain MC4100 (torR_ec⁺) carrying pPTor_so7 (torE"-lacZ) and strain LCB621 (torR_ec) carrying pPTor_so7 alone or with pR_so2 (torR_so⁺) were grown anaerobically in LB medium supplemented with glucose to limit torR_so expression, in the presence (+) or absence (−) of TMAO. β-Galactosidase activities of the plasmid-borne torE"-lacZ fusion are expressed in Miller units. (B) Autophosphorylation of the E. coli TorS726 sensor and transphosphorylation of the TorR response regulators from S. oneidensis and E. coli. TorS726 alone (lane 2) or with TorR_so (lane 1) or TorR_ec (lane 3) was incubated at room temperature for 10 min in the presence of [γ-³²P]ATP, and the reaction mixtures were analyzed on SDS-20% PAGE. The autoradiogram of the gel is shown. The positions on the gel of the TorR and TorS726 proteins are indicated.