TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| MC4100 | araD139 Δ(lacIPOZYA-argF)U169 rpsL thi | M. J. Casadaban |
| LCB621 | MC4100 torR49::mini-Tn 10 (Tcr) | 32 |
| BW26416 | BW25113 Δ(torSTRCAD) Kmr | 7 |
| LCB436 | Δ(torSTRCAD) Kmr derivative of MC4100 | This work |
| S. oneidensis ATCC 700550 | Wild type, formerly S. putrefaciens MR1 | 23 |
| Plasmids | ||
| pBAD24 | Vector containing P BAD promoter with a pBR origin of replication | 13 |
| pBAD33 | Vector containing P BAD promoter with a pACYC origin of replication | 13 |
| pGE593 | Operon fusion vector with a pBR origin of replication | 10 |
| pRSO1 | torR sequence from S. oneidensis inserted into pBAD24 | This work |
| pRSO2 | torR sequence from S. oneidensis inserted into pBAD33 | This work |
| pPTorSO4 | torE promoter (−481 to +217)a from S. oneidensis cloned into pGE593 | This work |
| pPTorSO7 | torE promoter (−84 to +119)a from S. oneidensis cloned into pGE593 | This work |
| pPTorSO9 | torE promoter (−60 to +119)a from S. oneidensis cloned into pGE593 | This work |
| pSTRSO | torSTR sequence from S. oneidensis cloned into pBAD33 | This work |
| pTRSO | pSTRSO but with torS partly deleted | This work |
| pSRSO | pSTRSO but with torT disrupted | This work |
| pSSO | pSTRSO but with torR and part of torT deleted | This work |
a
Nucleotide positions relative to the transcription start site of torE.