TABLE 3.
Promoter activities of xopA, xopD, and hpaF in X. campestris pv. vesicatoria strains 85-10 and 85*a
Reporter construct (gene) | Activity (GUS units) ± SDb in strain:
|
Fold induction (compared to 85-10) | |
---|---|---|---|
85-10 | 85*c | ||
pICxopA (xopA) | 1.2 ± 0.2 | 24 ± 3 | 20 |
pICxopD (xopD) | 0.24 ± 0.06 | 1.9 ± 0.4 | 8 |
pXV4::462 (hpaF) | 0.06 ± 0.02 | 2.4 ± 0.5 | 40 |
Bacteria were grown in NYG medium.
One unit corresponds to 1 nmol of 4-methylumbelliferone released per min per bacterium.
Carries the hrpG* mutation, which leads to constitutive hrp gene expression (45).