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Plating instructions for transformation reactions.
| Reaction | AD plasmid | BD plasmid | Plate type | Remarks |
|---|---|---|---|---|
| 1 | pNubG-Fe65 | pTSU2-APP | SD-TL, SD-TLH, SD-TLHA | Positive control |
| 2 | pPR3N | pTSU2-APP | SD-TL, SD-TLH, SD-TLHA | Negative control |
| 3 | pPR3N | pBT3STE-VP1 | SD-TL, SD-TLH, SD-TLHA | Self-activation detection |
| 4 | pOST1-NubI | pBT3STE-VP1 | SD-TL, SD-TLH, SD-TLHA | Functional test |
