TABLE 1.
Strain, plasmid, or phage | Parent strain | Relevant genotype and/or characteristics | Source or reference |
---|---|---|---|
Bacterial strains | |||
DJ901 | GC4468 | soxS::kan | 25 |
M542 | GC4468 | λRS45:rob2::lacZ kan | This study |
M564 | RK5173 | inaA::lacZ | This study |
M565 | RK12466 | inaA::lacZ | This study |
M597 | N8461 | pTA108 | This study |
M598 | N8461 | pTA108:marA | This study |
M599 | N8461 | pTA108:soxS | This study |
M600 | N8461 | pTA108:rob | This study |
M794 | BL21 (λDE3) | pET15b:rob | This study |
M808 | N8461 | pTA108:robΔ(370-867) | This study |
M848 | N8461 | pTA108:robΔ(778-867) | This study |
M851 | N8461 | pTA108:robΔ(643-867) | This study |
M853 | N8461 | pTA108:robΔ(529-867) | This study |
M854 | N8461 | pTA108:robΔ(451-867) | This study |
M871 | N7840 | marΔ sox-8::cat | 32; this study |
N7840 | GC4468 | marΔ Cams | 25 |
N7962 | N7840 | inaA::lacZ | 25 |
N7969 | DJ901 | soxS::kan marΔ inaA::lacZ | 25 |
N8461 | GC4468 | marΔ sox-8::cat rob::kan | This study |
N8695 | N7840 | mar::lacZ | 20 |
N9082 | N7840 | fpr::lacZ | 20 |
N9083 | N7840 | fumC::lacZ | 20 |
N9084 | N7840 | micF::lacZ | 20 |
N9085 | N7840 | nfo::lacZ | 20 |
N9086 | N7840 | sodA::lacZ | 20 |
N9087 | N7840 | zwf::lacZ | 20 |
RK5173 | MC4100 | 9 | |
RK12466 | MC4100 | tonB::kan | 9 |
Plasmids and phage | |||
pRS551 | pBR322 derivative; AmpR | 27 | |
pTA108 | pSC101 derivative; lac promoter; AmpR | 31 | |
λRS45 | λimm21; KanR | 27 | |
P1 cat clr-100 | Used for transductions | 25 |
See Materials and Methods for additional strains used.