TABLE 3.
Activation of rob regulon promoters by treatment with dipyridyla
| Strain (promoter-lacZ fusion) | β-Galactosidase activity (Miller units) with:
|
Ratiob | β-Galactosidase activity (Miller units) with 4,4"-dipyridyl | Ratiob | |
|---|---|---|---|---|---|
| No added dipyridyl | 5 mM 2,2"-dipyridyl | ||||
| N9082 (fpr) | 32 | 159 | 4.9 | 69 | 2.2 |
| N9083 (fumC) | 22 | 417 | 18.8 | 248 | 11.2 |
| N7962 (inaA) | 9.8 | 125 | 12.7 | 83 | 8.5 |
| N8695 (mar) | 344 | 1,117 | 3.2 | 912 | 2.7 |
| N9084 (micF) | 39 | 293 | 7.5 | 337 | 8.6 |
| N9085 (nfo) | 107 | 232 | 2.2 | 139 | 1.3 |
| N9086 (sodA) | 518 | 1,378 | 2.7 | 705 | 1.4 |
| N9087 (zwf) | 103 | 229 | 2.2 | 129 | 1.2 |
a
Derivatives of strain N7840 (Δmar) with the indicated single-copy promoter-lacZ transcriptional fusions were treated with 5 mM 2,2"- or 4,4"-dipyridyl for 1 h at 37°C and assayed for β-galactosidase.
b
That is, the ratio of β-galactosidase activities in the cells treated with dipyridyl to that in the untreated cells.