Skip to main content
. 2002 Mar;184(5):1407–1416. doi: 10.1128/JB.184.5.1407-1416.2002

TABLE 4.

2,2"-Dipyridyl affects the transcription of inaA and rob promoters differently depending on the growth phase of the cellsa

Strain (promoter-lacZ fusion) 2,2"-Dipyridyl concn (mM) or ratiob β-Galactosidase activity (Miller units) at various growth phases
Early log Mid log Stationary
M542 (rob) 0 240 469 732
5 396 507 741
Ratio 1.7 1.1 1.0
M871 (inaA) 0 16 16 20
5 89 131 119
Ratio 5.6 8.2 6.0
a

Cells were grown from small inoculae in LB broth to an A600 of 0.07 (early log phase) or 0.5 (mid-log phase) or overnight to an A600 of 2.5 (stationary phase). The samples were then diluted with an equal volume of prewarmed 10 mM 2,2"-dipyridyl in LB broth. After aeration for 1 h at 37°C, the β-galactosidase activity was assayed.

b

That is, the ratio of activities in the cells treated with dipyridyl to that in the untreated cells.