TABLE 4.
Strain (promoter-lacZ fusion) | 2,2"-Dipyridyl concn (mM) or ratiob | β-Galactosidase activity (Miller units) at various growth phases
|
||
---|---|---|---|---|
Early log | Mid log | Stationary | ||
M542 (rob) | 0 | 240 | 469 | 732 |
5 | 396 | 507 | 741 | |
Ratio | 1.7 | 1.1 | 1.0 | |
M871 (inaA) | 0 | 16 | 16 | 20 |
5 | 89 | 131 | 119 | |
Ratio | 5.6 | 8.2 | 6.0 |
Cells were grown from small inoculae in LB broth to an A600 of 0.07 (early log phase) or 0.5 (mid-log phase) or overnight to an A600 of 2.5 (stationary phase). The samples were then diluted with an equal volume of prewarmed 10 mM 2,2"-dipyridyl in LB broth. After aeration for 1 h at 37°C, the β-galactosidase activity was assayed.
That is, the ratio of activities in the cells treated with dipyridyl to that in the untreated cells.