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. 2002 Mar;184(5):1407–1416. doi: 10.1128/JB.184.5.1407-1416.2002

TABLE 6.

Effects of carboxyl-terminus deletions on activation of Rob by dipyridyla

Strain rob coding sequences present (bp) β-Galactosidase activity (Miller units):
Ratiob β-Galactosidase activity (Miller units) with 5 mM 4,4"-dipyridyl Ratiob
In untreated samples With 5 mM 2,2"-dipyridyl
M597 None 7.8 18 2.3 13 1.6
M600 1-867 (wild type) 21 392 18.3 149 6.9
M848 1-777 16 19 1.2 21 1.3
M851 1-642 29 23 0.80 25 0.87
M853 1-528 20 15 0.74 18 0.92
M854 1-450 30 32 1.1 19 0.74
M808 1-369 17 18 1.1 24 1.4
a

The mar sox rob mutant strains carrying the indicated plasmids were grown to early log phase, treated with 0 or 5 mM 2,2"- or 4,4"-dipyridyl for 1 h at 37°C, and assayed for β-galactosidase activity.

b

That is, the ratio of activities in the cells treated with 2,2"- or 4,4"-dipyridyl to that in the untreated cells.