TABLE 6.
Strain | rob coding sequences present (bp) | β-Galactosidase activity (Miller units):
|
Ratiob | β-Galactosidase activity (Miller units) with 5 mM 4,4"-dipyridyl | Ratiob | |
---|---|---|---|---|---|---|
In untreated samples | With 5 mM 2,2"-dipyridyl | |||||
M597 | None | 7.8 | 18 | 2.3 | 13 | 1.6 |
M600 | 1-867 (wild type) | 21 | 392 | 18.3 | 149 | 6.9 |
M848 | 1-777 | 16 | 19 | 1.2 | 21 | 1.3 |
M851 | 1-642 | 29 | 23 | 0.80 | 25 | 0.87 |
M853 | 1-528 | 20 | 15 | 0.74 | 18 | 0.92 |
M854 | 1-450 | 30 | 32 | 1.1 | 19 | 0.74 |
M808 | 1-369 | 17 | 18 | 1.1 | 24 | 1.4 |
The mar sox rob mutant strains carrying the indicated plasmids were grown to early log phase, treated with 0 or 5 mM 2,2"- or 4,4"-dipyridyl for 1 h at 37°C, and assayed for β-galactosidase activity.
That is, the ratio of activities in the cells treated with 2,2"- or 4,4"-dipyridyl to that in the untreated cells.