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Predicted membrane topology of PcaK. The model is based upon the algorithm of Jones et al. (11). Other algorithms yielded an almost identical topology. Predicted hydrophobic transmembrane segments are enclosed in boxes. The amino acid residues of PcaK that were mutagenized in this study are shaded various colors. The two conserved amino acid sequences in the 2-3 and 8-9 cytoplasmic loops are shaded grey.
